rad1 a1047 antibody Search Results


anti-rad1 polyclonal antibodies a1047  (ABclonal Biotechnology)
90
ABclonal Biotechnology anti-rad1 polyclonal antibodies a1047
Temporal regulation of the piRNA target genes Xrcc2 and <t>Rad1</t> is essential for sperm formation. (A) Dual-luciferase reporter assays to determine the effects of piR-mmu-170840 on the Xrcc2 reporter (left), and piR-mmu-604682 on the Rad1 reporter (right) in transfected GC-2spd(ts) cells. A mutant piRNA (Mut) served as negative control in each case. (B) Western blot analyses of Xrcc2 and Rad1 expression in enriched SC and RS, with β-actin serving as the loading control. (C , D) qRT-PCR (C) or northern blot (D) analyses of the mRNA levels of Xrcc2 and Rad1 in Miwi +/− and Miwi −/− mouse spermatogenic cells, with GAPDH serving as an internal control. Mean ± SEM of three separate experiments were plotted. Student's t -test significance: * P < 0.05, ** P < 0.01, *** P < 0.001. (E) PCR analyses of GFP DNA in extracted genomic DNA from late spermatids (top) and epididymal sperms (bottom) from mouse testes transduced with IRES-EGFP (lanes 4-8), Xrcc2-IRES-EGFP (lanes 10-14), or Rad1-IRES-EGFP (lanes 16-20), respectively. Genomic DNA samples from Oct4-GFP transgenic mice (lane 1) and wild-type background mice (lane 2) served as positive and negative controls, respectively. Results shown are representative of three independent experiments.
Anti Rad1 Polyclonal Antibodies A1047, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rad1+a1047+antibody/pmc04650574-174-0-7?v=ABclonal+Biotechnology
Average 90 stars, based on 1 article reviews
anti-rad1 polyclonal antibodies a1047 - by Bioz Stars, 2026-06
90/100 stars
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Temporal regulation of the piRNA target genes Xrcc2 and Rad1 is essential for sperm formation. (A) Dual-luciferase reporter assays to determine the effects of piR-mmu-170840 on the Xrcc2 reporter (left), and piR-mmu-604682 on the Rad1 reporter (right) in transfected GC-2spd(ts) cells. A mutant piRNA (Mut) served as negative control in each case. (B) Western blot analyses of Xrcc2 and Rad1 expression in enriched SC and RS, with β-actin serving as the loading control. (C , D) qRT-PCR (C) or northern blot (D) analyses of the mRNA levels of Xrcc2 and Rad1 in Miwi +/− and Miwi −/− mouse spermatogenic cells, with GAPDH serving as an internal control. Mean ± SEM of three separate experiments were plotted. Student's t -test significance: * P < 0.05, ** P < 0.01, *** P < 0.001. (E) PCR analyses of GFP DNA in extracted genomic DNA from late spermatids (top) and epididymal sperms (bottom) from mouse testes transduced with IRES-EGFP (lanes 4-8), Xrcc2-IRES-EGFP (lanes 10-14), or Rad1-IRES-EGFP (lanes 16-20), respectively. Genomic DNA samples from Oct4-GFP transgenic mice (lane 1) and wild-type background mice (lane 2) served as positive and negative controls, respectively. Results shown are representative of three independent experiments.

Journal: Cell Research

Article Title: MIWI and piRNA-mediated cleavage of messenger RNAs in mouse testes

doi: 10.1038/cr.2015.4

Figure Lengend Snippet: Temporal regulation of the piRNA target genes Xrcc2 and Rad1 is essential for sperm formation. (A) Dual-luciferase reporter assays to determine the effects of piR-mmu-170840 on the Xrcc2 reporter (left), and piR-mmu-604682 on the Rad1 reporter (right) in transfected GC-2spd(ts) cells. A mutant piRNA (Mut) served as negative control in each case. (B) Western blot analyses of Xrcc2 and Rad1 expression in enriched SC and RS, with β-actin serving as the loading control. (C , D) qRT-PCR (C) or northern blot (D) analyses of the mRNA levels of Xrcc2 and Rad1 in Miwi +/− and Miwi −/− mouse spermatogenic cells, with GAPDH serving as an internal control. Mean ± SEM of three separate experiments were plotted. Student's t -test significance: * P < 0.05, ** P < 0.01, *** P < 0.001. (E) PCR analyses of GFP DNA in extracted genomic DNA from late spermatids (top) and epididymal sperms (bottom) from mouse testes transduced with IRES-EGFP (lanes 4-8), Xrcc2-IRES-EGFP (lanes 10-14), or Rad1-IRES-EGFP (lanes 16-20), respectively. Genomic DNA samples from Oct4-GFP transgenic mice (lane 1) and wild-type background mice (lane 2) served as positive and negative controls, respectively. Results shown are representative of three independent experiments.

Article Snippet: Rabbit anti-Rad1 polyclonal antibodies (A1047) was from ABclonal Technology and anti-GFP (MBL-598) was from MBL.

Techniques: Luciferase, Transfection, Mutagenesis, Negative Control, Western Blot, Expressing, Control, Quantitative RT-PCR, Northern Blot, Transduction, Transgenic Assay